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balb cj mice aged  (Jackson Laboratory)

 
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    Jackson Laboratory balb cj mice aged
    In vivo efficacy of Vmb-9 in suppressing corneal neovascularization after alkali injury. ( A ) Representative images <t>from</t> <t>BALB/cJ</t> mice subjected to corneal alkali burn and treated with vehicle or Vmb-9 are shown at days 0, 1, 3, and 7 post-injury. Vehicle-treated eyes display progressive corneal neovascularization, whereas Vmb-9-treated eyes show marked attenuation of vascular ingrowth over time. For each time point, fluorescein staining ( top ) highlights epithelial integrity, ring light images ( middle ) document gross cornea integrity, and white-light images ( bottom ) show overall corneal clarity. Arrows indicated neovascularization in untreated mice. Right , Corneal whole-mount immunostaining for CD31 (blood vessels) and LYVE1 (lymphatic vessels) confirms a reduction in both hemangiogenesis and lymphangiogenesis in Vmb-9-treated corneas. ( B, C ) Quantitative analysis of CD31 + B and LYVE1 + C was performed using skeletonized images and analyzed with the Analyze Skeleton (2D/3D) plugin in Fiji (ImageJ, version 2.16.0), from which network parameters including the number of branches and number of junctions were extracted for each condition.
    Balb Cj Mice Aged, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/balb+cj+mice+aged/pmc13206737-69-0-8?v=Jackson+Laboratory
    Average 86 stars, based on 1 article reviews
    balb cj mice aged - by Bioz Stars, 2026-07
    86/100 stars

    Images

    1) Product Images from "Development of Miniprotein Binders to Inhibit VEGF-Induced Corneal Neovascularization"

    Article Title: Development of Miniprotein Binders to Inhibit VEGF-Induced Corneal Neovascularization

    Journal: Investigative Ophthalmology & Visual Science

    doi: 10.1167/iovs.67.5.61

    In vivo efficacy of Vmb-9 in suppressing corneal neovascularization after alkali injury. ( A ) Representative images from BALB/cJ mice subjected to corneal alkali burn and treated with vehicle or Vmb-9 are shown at days 0, 1, 3, and 7 post-injury. Vehicle-treated eyes display progressive corneal neovascularization, whereas Vmb-9-treated eyes show marked attenuation of vascular ingrowth over time. For each time point, fluorescein staining ( top ) highlights epithelial integrity, ring light images ( middle ) document gross cornea integrity, and white-light images ( bottom ) show overall corneal clarity. Arrows indicated neovascularization in untreated mice. Right , Corneal whole-mount immunostaining for CD31 (blood vessels) and LYVE1 (lymphatic vessels) confirms a reduction in both hemangiogenesis and lymphangiogenesis in Vmb-9-treated corneas. ( B, C ) Quantitative analysis of CD31 + B and LYVE1 + C was performed using skeletonized images and analyzed with the Analyze Skeleton (2D/3D) plugin in Fiji (ImageJ, version 2.16.0), from which network parameters including the number of branches and number of junctions were extracted for each condition.
    Figure Legend Snippet: In vivo efficacy of Vmb-9 in suppressing corneal neovascularization after alkali injury. ( A ) Representative images from BALB/cJ mice subjected to corneal alkali burn and treated with vehicle or Vmb-9 are shown at days 0, 1, 3, and 7 post-injury. Vehicle-treated eyes display progressive corneal neovascularization, whereas Vmb-9-treated eyes show marked attenuation of vascular ingrowth over time. For each time point, fluorescein staining ( top ) highlights epithelial integrity, ring light images ( middle ) document gross cornea integrity, and white-light images ( bottom ) show overall corneal clarity. Arrows indicated neovascularization in untreated mice. Right , Corneal whole-mount immunostaining for CD31 (blood vessels) and LYVE1 (lymphatic vessels) confirms a reduction in both hemangiogenesis and lymphangiogenesis in Vmb-9-treated corneas. ( B, C ) Quantitative analysis of CD31 + B and LYVE1 + C was performed using skeletonized images and analyzed with the Analyze Skeleton (2D/3D) plugin in Fiji (ImageJ, version 2.16.0), from which network parameters including the number of branches and number of junctions were extracted for each condition.

    Techniques Used: In Vivo, Staining, Immunostaining



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    In vivo efficacy of Vmb-9 in suppressing corneal neovascularization after alkali injury. ( A ) Representative images <t>from</t> <t>BALB/cJ</t> mice subjected to corneal alkali burn and treated with vehicle or Vmb-9 are shown at days 0, 1, 3, and 7 post-injury. Vehicle-treated eyes display progressive corneal neovascularization, whereas Vmb-9-treated eyes show marked attenuation of vascular ingrowth over time. For each time point, fluorescein staining ( top ) highlights epithelial integrity, ring light images ( middle ) document gross cornea integrity, and white-light images ( bottom ) show overall corneal clarity. Arrows indicated neovascularization in untreated mice. Right , Corneal whole-mount immunostaining for CD31 (blood vessels) and LYVE1 (lymphatic vessels) confirms a reduction in both hemangiogenesis and lymphangiogenesis in Vmb-9-treated corneas. ( B, C ) Quantitative analysis of CD31 + B and LYVE1 + C was performed using skeletonized images and analyzed with the Analyze Skeleton (2D/3D) plugin in Fiji (ImageJ, version 2.16.0), from which network parameters including the number of branches and number of junctions were extracted for each condition.
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    In vivo efficacy of Vmb-9 in suppressing corneal neovascularization after alkali injury. ( A ) Representative images <t>from</t> <t>BALB/cJ</t> mice subjected to corneal alkali burn and treated with vehicle or Vmb-9 are shown at days 0, 1, 3, and 7 post-injury. Vehicle-treated eyes display progressive corneal neovascularization, whereas Vmb-9-treated eyes show marked attenuation of vascular ingrowth over time. For each time point, fluorescein staining ( top ) highlights epithelial integrity, ring light images ( middle ) document gross cornea integrity, and white-light images ( bottom ) show overall corneal clarity. Arrows indicated neovascularization in untreated mice. Right , Corneal whole-mount immunostaining for CD31 (blood vessels) and LYVE1 (lymphatic vessels) confirms a reduction in both hemangiogenesis and lymphangiogenesis in Vmb-9-treated corneas. ( B, C ) Quantitative analysis of CD31 + B and LYVE1 + C was performed using skeletonized images and analyzed with the Analyze Skeleton (2D/3D) plugin in Fiji (ImageJ, version 2.16.0), from which network parameters including the number of branches and number of junctions were extracted for each condition.
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    In vivo efficacy of Vmb-9 in suppressing corneal neovascularization after alkali injury. ( A ) Representative images <t>from</t> <t>BALB/cJ</t> mice subjected to corneal alkali burn and treated with vehicle or Vmb-9 are shown at days 0, 1, 3, and 7 post-injury. Vehicle-treated eyes display progressive corneal neovascularization, whereas Vmb-9-treated eyes show marked attenuation of vascular ingrowth over time. For each time point, fluorescein staining ( top ) highlights epithelial integrity, ring light images ( middle ) document gross cornea integrity, and white-light images ( bottom ) show overall corneal clarity. Arrows indicated neovascularization in untreated mice. Right , Corneal whole-mount immunostaining for CD31 (blood vessels) and LYVE1 (lymphatic vessels) confirms a reduction in both hemangiogenesis and lymphangiogenesis in Vmb-9-treated corneas. ( B, C ) Quantitative analysis of CD31 + B and LYVE1 + C was performed using skeletonized images and analyzed with the Analyze Skeleton (2D/3D) plugin in Fiji (ImageJ, version 2.16.0), from which network parameters including the number of branches and number of junctions were extracted for each condition.
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    In vivo efficacy of Vmb-9 in suppressing corneal neovascularization after alkali injury. ( A ) Representative images <t>from</t> <t>BALB/cJ</t> mice subjected to corneal alkali burn and treated with vehicle or Vmb-9 are shown at days 0, 1, 3, and 7 post-injury. Vehicle-treated eyes display progressive corneal neovascularization, whereas Vmb-9-treated eyes show marked attenuation of vascular ingrowth over time. For each time point, fluorescein staining ( top ) highlights epithelial integrity, ring light images ( middle ) document gross cornea integrity, and white-light images ( bottom ) show overall corneal clarity. Arrows indicated neovascularization in untreated mice. Right , Corneal whole-mount immunostaining for CD31 (blood vessels) and LYVE1 (lymphatic vessels) confirms a reduction in both hemangiogenesis and lymphangiogenesis in Vmb-9-treated corneas. ( B, C ) Quantitative analysis of CD31 + B and LYVE1 + C was performed using skeletonized images and analyzed with the Analyze Skeleton (2D/3D) plugin in Fiji (ImageJ, version 2.16.0), from which network parameters including the number of branches and number of junctions were extracted for each condition.
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    In vivo efficacy of Vmb-9 in suppressing corneal neovascularization after alkali injury. ( A ) Representative images <t>from</t> <t>BALB/cJ</t> mice subjected to corneal alkali burn and treated with vehicle or Vmb-9 are shown at days 0, 1, 3, and 7 post-injury. Vehicle-treated eyes display progressive corneal neovascularization, whereas Vmb-9-treated eyes show marked attenuation of vascular ingrowth over time. For each time point, fluorescein staining ( top ) highlights epithelial integrity, ring light images ( middle ) document gross cornea integrity, and white-light images ( bottom ) show overall corneal clarity. Arrows indicated neovascularization in untreated mice. Right , Corneal whole-mount immunostaining for CD31 (blood vessels) and LYVE1 (lymphatic vessels) confirms a reduction in both hemangiogenesis and lymphangiogenesis in Vmb-9-treated corneas. ( B, C ) Quantitative analysis of CD31 + B and LYVE1 + C was performed using skeletonized images and analyzed with the Analyze Skeleton (2D/3D) plugin in Fiji (ImageJ, version 2.16.0), from which network parameters including the number of branches and number of junctions were extracted for each condition.
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    In vivo efficacy of Vmb-9 in suppressing corneal neovascularization after alkali injury. ( A ) Representative images <t>from</t> <t>BALB/cJ</t> mice subjected to corneal alkali burn and treated with vehicle or Vmb-9 are shown at days 0, 1, 3, and 7 post-injury. Vehicle-treated eyes display progressive corneal neovascularization, whereas Vmb-9-treated eyes show marked attenuation of vascular ingrowth over time. For each time point, fluorescein staining ( top ) highlights epithelial integrity, ring light images ( middle ) document gross cornea integrity, and white-light images ( bottom ) show overall corneal clarity. Arrows indicated neovascularization in untreated mice. Right , Corneal whole-mount immunostaining for CD31 (blood vessels) and LYVE1 (lymphatic vessels) confirms a reduction in both hemangiogenesis and lymphangiogenesis in Vmb-9-treated corneas. ( B, C ) Quantitative analysis of CD31 + B and LYVE1 + C was performed using skeletonized images and analyzed with the Analyze Skeleton (2D/3D) plugin in Fiji (ImageJ, version 2.16.0), from which network parameters including the number of branches and number of junctions were extracted for each condition.
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    In vivo efficacy of Vmb-9 in suppressing corneal neovascularization after alkali injury. ( A ) Representative images <t>from</t> <t>BALB/cJ</t> mice subjected to corneal alkali burn and treated with vehicle or Vmb-9 are shown at days 0, 1, 3, and 7 post-injury. Vehicle-treated eyes display progressive corneal neovascularization, whereas Vmb-9-treated eyes show marked attenuation of vascular ingrowth over time. For each time point, fluorescein staining ( top ) highlights epithelial integrity, ring light images ( middle ) document gross cornea integrity, and white-light images ( bottom ) show overall corneal clarity. Arrows indicated neovascularization in untreated mice. Right , Corneal whole-mount immunostaining for CD31 (blood vessels) and LYVE1 (lymphatic vessels) confirms a reduction in both hemangiogenesis and lymphangiogenesis in Vmb-9-treated corneas. ( B, C ) Quantitative analysis of CD31 + B and LYVE1 + C was performed using skeletonized images and analyzed with the Analyze Skeleton (2D/3D) plugin in Fiji (ImageJ, version 2.16.0), from which network parameters including the number of branches and number of junctions were extracted for each condition.
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    In vivo efficacy of Vmb-9 in suppressing corneal neovascularization after alkali injury. ( A ) Representative images <t>from</t> <t>BALB/cJ</t> mice subjected to corneal alkali burn and treated with vehicle or Vmb-9 are shown at days 0, 1, 3, and 7 post-injury. Vehicle-treated eyes display progressive corneal neovascularization, whereas Vmb-9-treated eyes show marked attenuation of vascular ingrowth over time. For each time point, fluorescein staining ( top ) highlights epithelial integrity, ring light images ( middle ) document gross cornea integrity, and white-light images ( bottom ) show overall corneal clarity. Arrows indicated neovascularization in untreated mice. Right , Corneal whole-mount immunostaining for CD31 (blood vessels) and LYVE1 (lymphatic vessels) confirms a reduction in both hemangiogenesis and lymphangiogenesis in Vmb-9-treated corneas. ( B, C ) Quantitative analysis of CD31 + B and LYVE1 + C was performed using skeletonized images and analyzed with the Analyze Skeleton (2D/3D) plugin in Fiji (ImageJ, version 2.16.0), from which network parameters including the number of branches and number of junctions were extracted for each condition.
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    Image Search Results


    In vivo efficacy of Vmb-9 in suppressing corneal neovascularization after alkali injury. ( A ) Representative images from BALB/cJ mice subjected to corneal alkali burn and treated with vehicle or Vmb-9 are shown at days 0, 1, 3, and 7 post-injury. Vehicle-treated eyes display progressive corneal neovascularization, whereas Vmb-9-treated eyes show marked attenuation of vascular ingrowth over time. For each time point, fluorescein staining ( top ) highlights epithelial integrity, ring light images ( middle ) document gross cornea integrity, and white-light images ( bottom ) show overall corneal clarity. Arrows indicated neovascularization in untreated mice. Right , Corneal whole-mount immunostaining for CD31 (blood vessels) and LYVE1 (lymphatic vessels) confirms a reduction in both hemangiogenesis and lymphangiogenesis in Vmb-9-treated corneas. ( B, C ) Quantitative analysis of CD31 + B and LYVE1 + C was performed using skeletonized images and analyzed with the Analyze Skeleton (2D/3D) plugin in Fiji (ImageJ, version 2.16.0), from which network parameters including the number of branches and number of junctions were extracted for each condition.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Development of Miniprotein Binders to Inhibit VEGF-Induced Corneal Neovascularization

    doi: 10.1167/iovs.67.5.61

    Figure Lengend Snippet: In vivo efficacy of Vmb-9 in suppressing corneal neovascularization after alkali injury. ( A ) Representative images from BALB/cJ mice subjected to corneal alkali burn and treated with vehicle or Vmb-9 are shown at days 0, 1, 3, and 7 post-injury. Vehicle-treated eyes display progressive corneal neovascularization, whereas Vmb-9-treated eyes show marked attenuation of vascular ingrowth over time. For each time point, fluorescein staining ( top ) highlights epithelial integrity, ring light images ( middle ) document gross cornea integrity, and white-light images ( bottom ) show overall corneal clarity. Arrows indicated neovascularization in untreated mice. Right , Corneal whole-mount immunostaining for CD31 (blood vessels) and LYVE1 (lymphatic vessels) confirms a reduction in both hemangiogenesis and lymphangiogenesis in Vmb-9-treated corneas. ( B, C ) Quantitative analysis of CD31 + B and LYVE1 + C was performed using skeletonized images and analyzed with the Analyze Skeleton (2D/3D) plugin in Fiji (ImageJ, version 2.16.0), from which network parameters including the number of branches and number of junctions were extracted for each condition.

    Article Snippet: BALB/cJ mice aged 6 weeks were obtained from The Jackson Laboratory (strain 000651; Bar Harbor, ME, USA) and allowed to acclimate for 1 week upon arrival.

    Techniques: In Vivo, Staining, Immunostaining